Background: Catheter-associated urinary tract infection (CAUTI) is a common nosocomial device-associated
infection, It is now recognized that the high infection rates were caused by the formation of biofilm on the surface
of the catheters that decreases the susceptibility to antibiotics and results in anti-microbial resistance.
In this study, we performed an in vitro test to explore the mechanism of biofilm formation and subsequently
conducted a multi-center clinical trial to investigate the efficacy of CAUTI prevention with the application of JUC, a
nanotechnology antimicrobial spray.
Methods: Siliconized latex urinary catheters were cut into fragments and sterilized by autoclaving. The sterilized
sample fragments were randomly divided into the therapy and control group, whereby they were sprayed with
JUC and distilled water respectively and dried before use.
The experimental standard strains of Escherichia coi (E. coll) were isolated from the urine samples of patients. At 16
hours and 7 days of incubation, the samples were extracted for confocal laser scanning microscopy.
A total of 1,150 patients were accrued in the clinical study. Patients were randomized according to the order of
surgical treatment. The odd array of patients was assigned as the therapy group (JUC), and the even amay of
patients was assigned as the control group (normal saline).
Results: After 16 hours of culture, bacterial biofilm formed on the surface of sample fragments from the control
group. In the therapy group, no bacterial biofilm formation was observed on the sample fragments. No significant
increase in bacterial colony count was observed in the therapy group after 7 days of incubation.
On the 7th day of catheterization, urine samples were collected for bacterial culture before extubation. Significant
difference was observed in the incidence of bacteriuria between the therapy group and control group (452% VS.
13.04%, p < 0.001).
Conclusions: In this study, the effectiveness of JUC in preventing CAUTI in a hospital setting was demonstrated in
both in vitro and clinical studies.
发布作者: Wei He, Dongmin Wang, Zhangqun Ye, Weihong Qian3
